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So I recently purchased a new fork for my fiancés bike as part of a gift I am putting together for our wedding but ran into an issue where the lower bearing on my headset won’t fit the fork taper.

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Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

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KM12 Cell Avalanche® Transfection Reagent (human colon adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies.

KM12 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

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I would an need an angular contact bearing with these measurements: 45.8mm outer diameter 39.8mm inner diameter 6.5mm depth 45x45 degree

A Reddit for Machinists of all varieties. From Old School conventional guys, to CNC Programmers, to the up and coming next generation.

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I had reached out to enduro bearings as they are Bay Area local but unfortunately they weren’t able to help. Hoping someone here can point me in the right direction!

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on KM12 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for KM12 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this KM12 Cell Avalanche® Transfection Reagent.

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on KM12 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for KM12 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this KM12 Cell Avalanche® Transfection Reagent.

KM12 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

KM12 Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on KM12 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for KM12 cells. No other transfection reagents can match the efficiency, convenience, and gentleness of KM12 Cell Avalanche® Transfection Reagent for transfection on KM12 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

KM12 Cell Avalanche® Transfection Reagent (human colon adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies.

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Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.