Myc Antibody (9E10) is an IgG1 κ mouse monoclonal Myc antibody (also designated c-Myc antibody) that detects the Myc protein of mouse, rat, human, monkey, feline and canine origin by WB, IP, IF, IHC(P), FCM and ELISA. c-Myc Antibody (9E10) is available as both the non-conjugated anti-c-Myc antibody form, as well as multiple conjugated forms of anti-c-Myc antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas. Therefore, the c-Myc Antibody (9E10) is an important reagent in cancer research. The presence of three sequence motifs in the c-Myc COOH terminus of the Myc protein, including the leucine zipper, the helix-loop-helix and a basic region, provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein, designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibits appreciable binding. Max can also form heterodimers with at least two additional bHLH-Zip proteins, Mad 1 and Mxi1, and Mad 1-Max dimers have been shown to repress transcription through interaction with mSin3

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz® conjugates which eliminates the need for a secondary antibody.Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Myc Antibody (9E10) has 9,750 citations in a variety of scientific publications.Primary antibodies like Anti-Myc Antibody (9E10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use. Myc Antibody (9E10) SpecificationsAlternate Product Name: MycProduct Clone Name: 9E10Classification: Primary AntibodyClonality: Monoclonal AntibodyAntibody Isotype: IgG1 kappa light chainAmino Acids: 408-439Epitope Target: c-MycEpitope Species: humanProduct Reactivity/Detection: c-Myc p67 and c-Myc tagged fusion proteinsSpecies Reactivity/Detection: mouse, rat, human, monkey, feline, canineAdditional Mammalian Reactivity: N/AAdditional Non-Mammalian Reactivity: N/AApplications: WB, IP, IF, IHC(P), FCM, ELISAVialing Information: Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatinResearch Citations: 9,750 citations in various scientific publicationsAvailable Conjugates/Forms for Myc Antibody (9E10): AC: Agarose, AF405: Alexa Fluor® 405, AF488: Alexa Fluor® 488, AF546: Alexa Fluor® 546, AF594: Alexa Fluor® 594, AF647: Alexa Fluor® 647, AF680: Alexa Fluor® 680, AF790: Alexa Fluor® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, PerCP: Peridinin Chlorophyll Protein Complex, PerCP: Peridinin Chlorophyll Protein Complex with Cyanin-5.5, TRITC: Rhodamine, X: TransCruz ReagentPrimary antibodies for non-mammalian targets are useful in detecting proteins of plant, viral, bacterial, avian, fish, yeast and invertebrates. Some examples include Drosophila, zebrafish, Xenopus, Arabidopsis, C. elegans, Clostridium, Campylobacter, Heliobacter pylori, Hepatitis, HIV, HPV, HSV, Influenza, Klebsiella, M. tuberculosis, Plasmodium, Pseudomonas, RSV, Saccharomyces cerevisiae, Salmonella, Schistosoma, Shigella and Staphylococcus.Santa Cruz Biotechnology monoclonal antibodies can be used in assays including flow cytometry, immunoprecipitation, Western blot analysis, and immunostaining. Anti-Myc Antibody (9E10) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded), FCM (Flow Cytometry), ELISA (Enzyme-Linked Immunosorbent Assay).Mouse secondary antibodies, control sera and control immunoglobulins are also offered to be used in combination with our primary monoclonal antibodies.Tissue extracts, nuclear extracts and whole cell lysates are used with Santa Cruz Biotechnology monoclonal antibodies for western blotting or immunoprecipitation positive controls. Nuclear extracts are ideal for western blot analysis or gel shift assays positive controls. Phospho-enriched whole cell lysates and tissue extracts are available for phospho-specific antibody positive controls.Monoclonal secondary antibodies are offered in various conjugated forms, including: Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), and TRITC (tetramethyl rhodamine isothiocyanate) (R), for research applications like Flow Cytometry (FCM), Immunofluorescence (IF), Immunohistochemistry (IHC) staining and Western Blotting (WB). Not intended for diagnostic or therapeutic use. Affinity purified immunoglobulins (IgGs)—conjugated and unconjugated—are available as negative controls for flow cytometry (FCM), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP) and Western Blotting (WB) applications.Normal sera for immunohistochemistry (IHC) applications are available as neat serum for blocking reagents. The serum which is used should match the species of the secondary antibody.

Myc Antibody (9E10) References:Transcriptional activation by the human c-Myc oncoprotein in yeast requires interaction with Max.  |  Amati, B., et al. 1992. Nature. 359: 423-6. PMID: 1406955Myc family oncoproteins function through a common pathway to transform normal cells in culture: cross-interference by Max and trans-acting dominant mutants.  |  Mukherjee, B., et al. 1992. Genes Dev. 6: 1480-92. PMID: 1644290Apoptosis and differentiation of human embryonic stem cells induced by sustained activation of c-Myc.  |  Sumi, T., et al. 2007. Oncogene. 26: 5564-76. PMID: 17369859Myc deletion rescues Apc deficiency in the small intestine.  |  Sansom, OJ., et al. 2007. Nature. 446: 676-9. PMID: 17377531Max: a helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex with Myc.  |  Blackwood, EM. and Eisenman, RN. 1991. Science. 251: 1211-7. PMID: 2006410Enhanced expression of the N-myc gene in Wilms' tumors.  |  Nisen, PD., et al. 1986. Cancer Res. 46: 6217-22. PMID: 2430692L-myc, a new myc-related gene amplified and expressed in human small cell lung cancer.  |  Nau, MM., et al. Nature. 318: 69-73. PMID: 2997622Homogeneously staining chromosomal regions contain amplified copies of an abundantly expressed cellular oncogene (c-myc) in malignant neuroendocrine cells from a human colon carcinoma.  |  Alitalo, K., et al. 1983. Proc Natl Acad Sci U S A. 80: 1707-11. PMID: 6300869Mad-Max transcriptional repression is mediated by ternary complex formation with mammalian homologs of yeast repressor Sin3.  |  Ayer, DE., et al. 1995. Cell. 80: 767-76. PMID: 7889570