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Protein glycosylation has been shown to have a variety of site-specific and glycan-specific effects, but so far, the molecular logic that leads to such observations has been elusive. Understanding the structural changes that occur and being able to correlate those with the physical properties of the glycopeptide are valuable steps toward being able to predict how specific glycosylation patterns will affect the stability of glycoproteins. By systematically comparing the structural features of the O-glycosylated carbohydrate-binding module of a Trichoderma reesei-derived Family 7 cellobiohydrolase, we were able to develop a better understanding of the influence of O-glycan structure on the molecule's physical stability. Our results indicate that the previously observed stabilizing effects of O-glycans come from the introduction of new bonding interactions to the structure and increased rigidity, while the decreased stability seemed to result from the impaired interactions and increased conformational flexibility. This type of knowledge provides a powerful and potentially general mechanism for improving the stability of proteins through glycoengineering.

A CV joint is a type of joint that allows the shaft to transmit power while it’s at an angle. It’s most commonly used in drivetrains, where it connects the rotating driveshaft to the non-rotating wheel axles.

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This movement allows the shaft to transmit power to the wheels while the suspension is moving up and down, always keeping the wheels in contact with the ground.

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In some cases, a bad wheel bearing can also cause the affected wheel to wobble or shake. This is usually most noticeable at higher speeds (above 40 MPH) and can be dangerous if not addressed quickly.

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Another symptom to watch out for is a vibration coming from the affected wheel. This is usually most noticeable at higher speeds (above 40 MPH) and can be dangerous if not addressed quickly.

CV joints are located at either end of the driveshaft and are usually covered by a rubber or plastic “boot” that protects them from dirt and debris.

Structural Insight into the Stabilizing Effect of O-Glycosylation.,Chaffey PK, Guan X, Chen C, Ruan Y, Wang X, Tran AH, Koelsch TN, Cui Q, Feng Y, Tan Z Biochemistry. 2017 Jun 13;56(23):2897-2906. doi: 10.1021/acs.biochem.7b00195., Epub 2017 Jun 1. PMID:28494147[1]

Wheel bearings are typically made up of an inner and outer ring, with the cage and balls in between. The inner ring is attached to the hub, while the outer ring is what the wheel bolts onto.

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Over time, however, wheel bearings can wear out, causing noise and vibration that can be quite noticeable while driving. In some cases, a failed wheel bearing can even cause the wheel to come loose from the vehicle while you’re driving!

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As the name implies, wheel bearings provide smooth, friction-free rotation to your wheels while you’re driving down the road. Without them, your wheels would literally grind to a halt.

Properly lubricated and adjusted wheel bearings will give you many miles of service. While improperly adjusted wheel bearings can fail in just a few short miles ...

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If you’re experiencing strange noises or vibrations while driving, there’s a good chance that you have a bad wheel bearing or CV joint. Identifying the cause can seem difficult, but there are a few ways to pinpoint the faulty part.

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The CV joint consists of two main parts: the “inner race” and the “outer race.” The inner race is attached to the shaft, while the outer race is attached to the wheel axle.

One of the most common symptoms is a clicking noise coming from the affected wheel when turning. This noise is caused by the balls inside the CV joint hitting against each other as they slide back and forth.

One of the most common symptoms is noise coming from the wheels or suspension area. This noise is usually described as a grinding, growling, or rumbling sound and is most noticeable when driving at lower speeds.

Between these two races are a number of small metal balls (called “balls”) that rotate along with the shaft. As the shaft turns, the balls slide back and forth inside the races, allowing the inner and outer races to move relative to each other.

Another symptom to watch out for is increased vibration coming from the affected wheel. This can be felt through the floorboard or steering wheel and is usually worst between 15-25 MPH.

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Most vehicles have four-wheel bearings – one at each corner of the vehicle – that support the weight of the vehicle and allow the wheels to rotate freely.

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To tell the difference between a bad wheel bearing and a bad CV joint, listen to the noise. If you hear a grinding, growling, or rumbling noise coming from the affected wheel, it’s a bad wheel bearing. If you hear a clicking noise coming from the affected wheel when turning, it’s a bad CV joint.

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A wheel bearing is a set of steel balls held together by a metal ring called a “cage.” The cage keeps the balls spaced evenly around the circumference of the bearing and prevents them from coming into contact with each other during operation.