In this webinar, we will provide an overview of ACD’s RNAscope™ in situ hybridization (ISH) technology and share considerations for non-common species, including Molly’s successful integration of these custom probes into her thesis research focused on prenatal THC exposure in ferrets. After an overview of RNAscope and the custom probe design process, Molly will outline her research parameters and rationale for using RNAscope, discuss experimental design and implementation of custom probes into her workflow, share imagery generated and insights on the custom probe request form and process.

RNAscope Kit price

Targeted gene therapy has shown great promise for treating rare diseases, particularly those that arise from single gene defects. Techniques to introduce therapeutic transgenes include adeno-associated virus (AAV), lentivirus, or lipid nanoparticle (LNP) mediated delivery. While qPCR-based methods provide average values for copy number from extracted nucleic acids, the RNAscope in situ hybridization (ISH) technology provides morphology-based, cell-specific quantification of vector DNA and transgene mRNA.

Speakers: Molly Pruitt Neuroscience Ph.D. Candidate University of Maryland, Baltimore. and Kymberly Gustus, Ph.D. Technical Support Scientist - Probe Design Advanced Cell Diagnostics, a Bio-Techne brand.

ACDRNAscope

ACDBio's TSA Vivid Fluorophore Kits are engineered for increased brightness and improved performance with tyramide signal amplification in IHC, ICC and FISH applications. They have been specifically optimized for exceptional signal-to-noise performance in the RNAscope™ Multiplex Fluorescent v2 Assay, enabling visualization of gene expression at the single cell level.

Learning Objectives:- General understanding of design strategies for different assay types- Workflow and application of made-to-order probe designs for detection in rare model species- Overview and guidance for New Probe Request (NPR) form fields and drop-down options

ACD’s products and services are based on its proprietary RNAscope technology – the first multiplex fluorescent and chromogenic in situ hybridisation platform, capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity.

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Image: Three-plex detection of glutaminergic neurons, Vglut (Red), Vglut2 (Green) and GABAergic neurons Vgat (White) expression in FFPE mouse brain with RNAscope Multiplex Florescent Assay V2. Nuclei were labelled with DAPI (Blue).

Advanced Cell Diagnostics, Inc. (ACD) is a leader in the emerging field of molecular pathology, developing cell- and tissue-based diagnostic tests for personalised medicine. Based in the heart of Silicon Valley, ACD was founded and managed by experienced entrepreneurs in the life science industry.

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RNAscope® Technology is a novel in situ hybridization (ISH) assay for detection of target RNA within intact cells. The assay represents a major advance in RNA ISH approaches with its proprietary probe design to amplify target-specific signals but not background noise from non-specific hybridization.

Advanced Cell Diagnostics, a Bio-Techne BrandJoin ACD’s Custom Probe Design Team and UMB Neuroscience PhD Candidate Molly Pruitt as they share insights into the design and application of custom RNA probes for specific and sensitive detection of a variety of targets across many species.

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