Congratulations on acquiring a set of Bones ®Bearings, known world-wide as the best brand of skateboard bearings available. All Bones® Bearings are Skate Rated™ to our precision “tolerances” to give you the absolute maximum speed and life possible, not “ABEC” rated like bearings designed to operate in motors or normal machinery. The superb quality of Bones® chromium bearing steel races with their mirror like “super finish,” removable, non-contact rubber shields, high speed, precision molded ball retainers, and Bones Speed Cream® lubricant provide unequalled roll and ease of maintenance. So, carefully install them in your wheels and you’re ready to roll.

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Immunofluorescent analysis of HeLa cells labeling Lamin A + C with ab190380 at 1/1000 dilution (red) and a chicken polyclonal antibody to Vimentin (green) and DNA (blue).

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4 combination 4 is equal to

Lamin A/C proteins play a role in maintaining nuclear stability chromosome organization and gene regulation. They are part of a complex network within the nuclear lamina that includes interactions with proteins and DNA. Lamin A with a molecular weight distinct from other lamins participates in assembling this supportive matrix and contributes to DNA maintenance and repair processes. Their interaction with chromatin and gene expression regulation emphasizes their influence on important cellular functions.

4C2

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Lamin-A/CLamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:2344612, PubMed:2188730, PubMed:24741066, PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:31434876, PubMed:31548606, PubMed:37788673, PubMed:37832547). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:24741066, PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:31548606, PubMed:37788673, PubMed:37832547). Lamin A and C also regulate matrix stiffness by conferring nuclear mechanical properties (PubMed:25127216, PubMed:23990565). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:2344612, PubMed:2188730). Lamin A and C are present in equal amounts in the lamina of mammals (PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:31548606). Also invoved in DNA repair: recruited by DNA repair proteins XRCC4 and IFFO1 to the DNA double-strand breaks (DSBs) to prevent chromosome translocation by immobilizing broken DNA ends (PubMed:31548606). Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation (PubMed:10080180, PubMed:10814726, PubMed:11799477, PubMed:18551513, PubMed:22431096). Required for osteoblastogenesis and bone formation (PubMed:12075506, PubMed:15317753, PubMed:18611980). Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone (PubMed:10587585). Required for cardiac homeostasis (PubMed:10580070, PubMed:12927431, PubMed:23666920, PubMed:18611980).Prelamin-A/CPrelamin-A/C can accelerate smooth muscle cell senescence (PubMed:20458013). It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence (PubMed:20458013).

Mutations in lamin A/C are linked to disorders such as Hutchinson-Gilford Progeria Syndrome and Emery-Dreifuss Muscular Dystrophy. These conditions highlight the importance of lamin A/C in cellular stability and nuclear integrity. Proteins such as emerin often relate to lamin A/C in these diseases as disruptions to their interactions can lead to compromised nuclear function and disease phenotypes.

4c4calculator

4c4multiplication

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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.

4c4permutation

Lanes 1 - 4: Merged signal (red and green). Green - ab190380 observed at 70-75 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.

ab190380 was shown to react with LMNA in wild-type HAP1 cells in Western blot. Loss of signal was observed when LMNA knockout sample was used. HAP1 wild-type and LMNA knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab190380 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

4c4examples

Your new Bones® Bearings are the most accurately manufactured part of your skateboard, with clearances and tolerances considerably smaller than 1/1000th of an inch. That means that a single hair is thicker than the clearance between the balls and the races inside the bearing, and a hair will clog it up and slow you down. Because you skateboard outside through dust, sand, water, mud, etc; your bearings will eventually get dirty and/or wet. When they are dirty, you will notice they don’t spin freely when you flick the wheel and you may also notice they are getting noisy. At this point you have two options: clean your bearings or replace them.

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High precision bearings like Bones® roll their best after about an hour of use in a clean environment (this initial use is called a “break in period”). If you want your bearings at their peak prior to a competition or a race, we highly recommend you break in your bearings, then clean and re-lubricate them and store in a moisture free container until you are ready to use them. When storing your bearings, be sure there is a light coat on the outer races with our Bones Speed Cream®, or a light oil to prevent them from rusting.

Note: We have attempted to foresee any possible problem you may have during your cleaning process, and so these instructions may seem complex at first reading. The bearing cleaning process is logical and simple though, and you should have no problem doing this if you can understand our sequential steps.

Mouse Monoclonal Lamin-A/C antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LMNA.

4P4

Mouse Monoclonal Lamin-A/C antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LMNA.

Lamin A/C proteins engage in the mechanosensory signaling and DNA damage response pathways. They interact with pathways involving the nuclear envelope structure and have connections to proteins like emerin and nuclear actin. Lamin A's role in these pathways supports its involvement in responding to mechanical stress and preserving genomic integrity highlighting its integration with these cellular processes.

WARNING! Don’t continue to use bearings that don’t spin freely on your skateboard. If you continue to ride very dirty or un-lubricated bearings, you run the risk of permanently damaging them and/or yourself. Dirt in your bearings will cause pitting of your balls and races, increase friction, cause extra heat, and can eventually cause the bearing to “freeze up” or stop rolling. If this happens to your bearings, the skateboard will stop abruptly, causing you to fall and be injured. So keep them clean, dry and lubricated with Speed Cream.

4C3

We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Lamin A + C (red) using ab190380 at 1/2000 dilution in ICC/IF. HSP60 is detected with an anti-HSP60 antibody (green). The nuclear counter stain is DAPI (blue).

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Lamin A and Lamin C also known as lamin A/C are proteins encoded by the LMNA gene. These proteins are key components of the nuclear envelope where they provide structural support and maintain the shape of the nucleus. The lamin A/C molecule has a molecular weight of approximately 60-70 kDa. Expression of lamin A and lamin C occurs predominantly in differentiated cells where these proteins integrate into the nuclear lamina alongside other lamin molecules like lamin B. Lamin A alone sometimes referred to by designations like 4C11 plays a significant role in mechanical support at a molecular level.