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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz® conjugates which eliminates the need for a secondary antibody.Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Y14 Antibody (4C4) has 12 citations in a variety of scientific publications.Primary antibodies like Anti-Y14 Antibody (4C4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use. Y14 Antibody (4C4) SpecificationsAlternate Product Name: Y14Product Clone Name: 4C4Classification: Primary AntibodyClonality: Monoclonal AntibodyAntibody Isotype: IgG2b kappa light chainAmino Acids: Epitope Target: Y14Epitope Species: humanProduct Reactivity/Detection: Y14 of human and Xenopus laevis origin, and RBM8 of mouse and rat originSpecies Reactivity/Detection: mouse, rat, humanAdditional Mammalian Reactivity: N/AAdditional Non-Mammalian Reactivity: XenopusApplications: WB, IP, IF, IHC(P), ELISAVialing Information: Each vial contains 200 µg IgG2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatinResearch Citations: 12 citations in various scientific publicationsAvailable Conjugates/Forms for Y14 Antibody (4C4): AC: Agarose, AF488: Alexa Fluor® 488, AF546: Alexa Fluor® 546, AF594: Alexa Fluor® 594, AF647: Alexa Fluor® 647, AF680: Alexa Fluor® 680, AF790: Alexa Fluor® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: PhycoerythrinPrimary antibodies for non-mammalian targets are useful in detecting proteins of plant, viral, bacterial, avian, fish, yeast and invertebrates. Some examples include Drosophila, zebrafish, Xenopus, Arabidopsis, C. elegans, Clostridium, Campylobacter, Heliobacter pylori, Hepatitis, HIV, HPV, HSV, Influenza, Klebsiella, M. tuberculosis, Plasmodium, Pseudomonas, RSV, Saccharomyces cerevisiae, Salmonella, Schistosoma, Shigella and Staphylococcus.Santa Cruz Biotechnology monoclonal antibodies can be used in assays including flow cytometry, immunoprecipitation, Western blot analysis, and immunostaining. Anti-Y14 Antibody (4C4) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded), ELISA (Enzyme-Linked Immunosorbent Assay).Mouse secondary antibodies, control sera and control immunoglobulins are also offered to be used in combination with our primary monoclonal antibodies.Tissue extracts, nuclear extracts and whole cell lysates are used with Santa Cruz Biotechnology monoclonal antibodies for western blotting or immunoprecipitation positive controls. Nuclear extracts are ideal for western blot analysis or gel shift assays positive controls. Phospho-enriched whole cell lysates and tissue extracts are available for phospho-specific antibody positive controls.Monoclonal secondary antibodies are offered in various conjugated forms, including: Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), and TRITC (tetramethyl rhodamine isothiocyanate) (R), for research applications like Flow Cytometry (FCM), Immunofluorescence (IF), Immunohistochemistry (IHC) staining and Western Blotting (WB). Not intended for diagnostic or therapeutic use. Affinity purified immunoglobulins (IgGs)—conjugated and unconjugated—are available as negative controls for flow cytometry (FCM), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP) and Western Blotting (WB) applications.Normal sera for immunohistochemistry (IHC) applications are available as neat serum for blocking reagents. The serum which is used should match the species of the secondary antibody.

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Y14 Antibody (4C4) References:MAGOH interacts with a novel RNA-binding protein.  |  Zhao, XF., et al. 2000. Genomics. 63: 145-8. PMID: 10662555Cloning and gene expression of a novel human ribonucleoprotein.  |  Conklin, DC., et al. 2000. Biochim Biophys Acta. 1492: 465-9. PMID: 11004516Nuclear mRNA binding proteins couple pre-mRNA splicing and post-splicing events.  |  Kim, VN. and Dreyfuss, G. 2001. Mol Cells. 12: 1-10. PMID: 11561715Drosophila Y14 shuttles to the posterior of the oocyte and is required for oskar mRNA transport.  |  Hachet, O. and Ephrussi, A. 2001. Curr Biol. 11: 1666-74. PMID: 11696323Magoh, a human homolog of Drosophila mago nashi protein, is a component of the splicing-dependent exon-exon junction complex.  |  Kataoka, N., et al. 2001. EMBO J. 20: 6424-33. PMID: 11707413The protein Mago provides a link between splicing and mRNA localization.  |  Le Hir, H., et al. 2001. EMBO Rep. 2: 1119-24. PMID: 11743026Translation is required to remove Y14 from mRNAs in the cytoplasm.  |  Dostie, J. and Dreyfuss, G. 2002. Curr Biol. 12: 1060-7. PMID: 121216125' exon interactions within the human spliceosome establish a framework for exon junction complex structure and assembly.  |  Reichert, VL., et al. 2002. Genes Dev. 16: 2778-91. PMID: 12414731

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Y14 Antibody (4C4) is an IgG2b κ mouse monoclonal Y14 antibody (also designated Y14 antibody) that detects the Y14 protein of human and Xenopus laevis origin, and RBM8 protein of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA. Y14 Antibody (4C4) is available as both the non-conjugated anti-Y14 antibody form, as well as multiple conjugated forms of anti-Y14 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The exon junction complex (EJC) is a multiprotein complex that assembles approximately 20-24 nucleotides upstream of exon-exon junctions in pre-mRNAs. It is involved in mRNA export, cytoplasmic localization, and nonsense-mediated mRNA decay. Members of the EJC include Y14, Aly/REF, Magoh, RNPS1, SRm160, and DEK. Aly/REF, Magoh, and Y14, identified as RBM8 in mouse and rat, make up the core of the EJC, and these proteins remain stably bound to spliced mRNAs in the cytoplasm until they are translated. Therefore, Y14, Aly/REF, and Magoh have the ability to communicate to the cytoplasm the processing history of the mRNA, including the position of the removed introns. The gene encoding human Y14 encodes three transcripts. Y14 is a ubiquitously expressed protein. Although Y14 shuttles to the cytoplasm, it is predominantly detected in the nucleus and is co-localized with oskar mRNA at the posterior pole of the cell.

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1. By using lubricants: friction increases as the roughness of the surface increases. Lubricants fill the fine pores and gaps between contact surfaces and reduces friction

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3. By using ball bearings: Rotating machines can be mounted on ball bearings. Rolling friction is lesser than sliding friction

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